|
Table
15 Inhibition of hemagglutination of GFL by mono- and oligosaccharides and
glycoproteins |
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|
Inhibitor a |
Minimun
inhibitor concentration (mM)b |
Specificity
factor c |
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|
Methyl
β - N-acetylgalactosamine |
0.104 |
1.00 |
||
|
Methyl
α - N-acetylgalactosamine |
0.125 |
1.20 |
||
|
N-acetylgalactosamine |
0.292 |
2.81 |
||
|
Methyl
β - galactoside |
1.88 |
18.1 |
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|
Galactose |
3.33 |
32.0 |
||
|
Fucose |
3.33 |
32.0 |
||
|
Methyl
α - galactoside |
5.00 |
48.1 |
||
|
Galactosamine |
12.5 |
120 |
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|
L-Arabinose |
35.3 |
339 |
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|
Methyl
α - N-acetylglucosamine |
43.6 |
419 |
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|
N-Acetylglucosamine |
50.1 |
482 |
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|
Glucosamine |
66.2 |
637 |
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|
Methyl
β - glucoside |
67.8 |
652 |
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|
L-Rhamnose |
78.7 |
757 |
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|
Xylose |
83.5 |
803 |
||
|
|
|
|
||
|
Lactulose
[β Gal (1-->4) Fru] |
1.66 |
16.0 |
||
|
N-Acetyllactosamine
[β Gal (1-->4) GlcNAc] |
2.04 |
19.6 |
||
|
Lactose
[β Gal (1-->4) Glc] |
2.92 |
28.1 |
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|
Melibiose
[αGal (1-->6) Glc] |
3.33 |
32.0 |
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|
Raffinose
[αGal (1-->6) αGlc(1-->2) βFru] |
6.66 |
64.0 |
||
|
|
|
|
||
|
GalNAc
[α (1-->4) GalNAc]n
n=1 |
0.292 |
2.81 |
||
|
|
2 |
0.292 |
2.81 |
|
|
|
3 |
0.292 |
2.81 |
|
|
|
4 |
0.292 |
2.81 |
|
|
|
5 |
0.292 |
2.81 |
|
|
|
6 |
0.292 |
2.81 |
|
|
|
( µ g/ml ) |
|
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|
Asialo-BSM |
0.00514 |
|
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|
BSM |
0.0302 |
|
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|
asialofetuin |
26 |
|
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|
fetuin |
2080 |
|
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|
|
|
|
|
|
|
a
Mannose, fructose, ribose, arabinose, glucose, mannosamine, methyl β
- N-acetylglucosaminide, methyl β - glucoside, and maltose did
not inhibit at all at concentrations up to 200 mM.
GalNH2 [α (1-->4) GalNH2]n
(n=1-7) and GalNAc [α (1-->4) GalNAc]7
did not exhinit at all at concentrations up to mM. |
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|
b
These are minimum concentration required for inhibition of
hemagglutination of GFL (titer 4) |
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|
c
Calculated in relation to methyl β - N-galactosaminide. |
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